5-(4,6-Dichloro-s-triazin-2-ylamino)fluorescein (hydrochloride)

5-(4,6-Dichloro-s-triazin-2-ylamino)fluorescein (DTAF) is an amine-reactive fluorescent probe for labeling proteins. It displays excitation/emission maxima of 492/513 nm, respectively. DTAF has been conjugated to IgG for use as fluorescent antibodies and as a pre-column or on-column reagent to label amino acids and amino acid-containing compounds. It has also been used as a derivatizing agent in the analysis of ephedrine and pseudoephedrine from various sources.Formal Name: 5-[(4,6-dichloro-1,3,5-triazin-2-yl)amino]-3',6'-dihydroxy-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one, monohydrochloride. CAS Number: 21811-74-5. Synonyms: Dichlorotriazinylaminofluorescein, DTAF. Molecular Formula: C23H12Cl2N4O5 . HCl. Formula Weight: 531.7. Purity: >98%. Emission: 513 nm. Excitation: 492 nm. Formulation: (Request formulation change), A solid. Solubility: DMF: 1 mg/ml, DMSO: 1 mg/ml, Ethanol: 1 mg/ml, PBS (pH 7.2): insol. lambdamax: 211, 225, 277 nm. SMILES: OC1=CC=C2C3(C4=CC=C(NC5=NC(Cl)=NC(Cl)=N5)C=C4C(O3)=O)C6=CC=C(O)C=C6OC2=C1.Cl. InChi Code: InChI=1S/C23H12Cl2N4O5.ClH/c24-20-27-21(25)29-22(28-20)26-10-1-4-14-13(7-10)19(32)34-23(14)15-5-2-11(30)8-17(15)33-18-9-12(31)3-6-16(18)23,/h1-9,30-31H,(H,26,27,28,29),1H. InChi Key: TZGKHPXUAULWOC-UHFFFAOYSA-N.