3,3'-Diethyloxacarbocyanine iodide

3,3'-Diethyloxacarbocyanine iodide is a cyanine based dye that has been used for measuring membrane potentials in bacteria. The green fluorescent dye forms red fluorescent aggregates with increasing membrane potential. The spectral shifting phenomenon has been exploited to measure membrane potential and analyze bacterial viability by flow cytometry ratiometrically. Spectral data: lambdamax 483nm (methanol), lambdaem 498nm (methanol). The dye has also been used as laser dye and in photosensitization of mitochondria and endoplastic recticulum in animals and plant cells. It may be used as a conjugate for labelling cells for cytofluorometry studies and in the imaging of carbon nanotubes(CNTs) using fluorescence microscopy. References: (1) D.N. Dempster, et al., J. Chem. Soc. Faraday II 68, 1479 (1972) , (2) D. Magde & M.W. Windsor, Chem. Phys. Lett. 27, 31 (1974) , (3) M. Zalokar & C. Sardet, Dev. Biol. 102, 195 (1984) , (4) M. Castedo, et al., J. Immunol. Meth. 265, 39 (2002) , (5) H.J. Broxterman, et al., Leukemia 11, 1110 (1997) , (6) D.J. Novo, et al., Cytometry 35, 55 (1999) , (7) D.J. Novo, et al., Antimicrob. Agents Chemother. 44, 827 (2000) , (8) D.R. Gentry, et al., J. Microbiol. Methods 83, 254 (2010) , (9) R.W. Sabnis, Handbook of biological dyes and stains (2010) , (10) M.A. Hudson, et al., Antimicrob. Agents Chemother. 64, e00910 (2020) Appearance: Dark red crystals or powder. Solubility: Soluble in DMSO or methanol. Identity: Determined by 1H-NMR. Declaration: Manufactured by Chemodex. Inchi Key: FIZZUEJIOKEFFZ-UHFFFAOYSA-M Smiles: CCN1/C(OC2=CC=CC=C12)=C\C=C\C3=[N+](CC)C4=CC=CC=C4O3.[I-]