2',7'-Dichlorofluorescein diacetate

Dichlorofluorescin diacetate (H2DCFDA) is a chemically reduced analog of fluorescein, used as an indicator for reactive oxygen species (ROS) in cells. The cell permeable non-fluorescent H2DCFDA is cleaved by cellular esterases and oxidation, and converted to the highly fluorescent 2',7'-dichlorofluorescein. H2DCFDA might be reactive toward a broad range of oxidizing reactions that may be increased during intracellular oxidant stress. Peroxynitrite is an efficient mediator of this oxidation and neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DCFH. This probe is widely used to monitoring cellular redox processes. Used as a component/substrate of the 2',7'-dichlorofluorescein diacetate (DCFH-DA) assay to quantitate reactive oxygen species (ROS). Can detect reactive oxygen species in live cells. Spectral data: lambdaEx=504nm/lambdaEm=529nm. Product References: (1) A.S. Ferrer, et al., Anal. Biochem. 187, 129 (1990) , (2) C.P. LeBel, et al., Toxicol. Appl. Pharmacol. 104, 17 (1990) , (3) A.R. Rosenkranz, et al., J. Immunol. Meth. 156, 39 (1992) , (4) F. Perez-Garcia, et al., Life Sci. 59, 2033 (1996) , (5) J.P. Crow, Nitric Oxide 1, 145 (1997) , (6) N.W. Kooy, et al., Free Rad. Res. 27, 245 (1997) , (7) C. Gabriel, et al., J. Pharmacol. Toxicol. Meth. 38, 93 (1997) , (8) O. Myhre, et al., Biochem. Pharmacol. 65, 1575 (2003) , (9) D. Figueroa, et al., J. Pharmacol. Toxicol. Meth. 94, 26 (2018) Appearance: White to off-white powder. Solubility: Soluble in DMSO (30mg/ml) or ethanol(5mg/ml). Identity: Determined by 1H-NMR. InChi Key: PXEZTIWVRVSYOK-UHFFFAOYSA-N Smiles: ClC1=C(OC(C)=O)C=C2C(C(C3=CC=CC=C3C(O)=O)C(C=C(Cl)C(OC(C)=O)=C4)=C4O2)=C1