1,1'-Dimethyl-3,3,3',3'-tetramethyl indocarbocyanine iodide

DiIC1(3) is a fluorescent carbocyanine dye used mainly for assessing membrane potential in mitochondria and plasma membranes, especially in flow cytometry and fluorescence microscopy. DiIC1(3) has short alkyl chains (methyl groups), so it diffuses more easily but is less stably anchored in membranes than longer-chain versions like DiIC12(3) or DiIC18(3). It is ideal for dynamic membrane potential assessments, not long-term labeling. The dye can be used as a non-reactive fluorophore, for control experiments and for calibration. Spectral Data: Excitation max: ~ 555nm, Emission max: ~570nm (accord. Lit).