n-Octyl-beta-D-glucopyranoside (ultrapure)

Non-ionic detergent for the functional solubilization, isolation, purification and crystallograpy of membrane proteins. Mild, non-denaturing detergent that is used for the solubilization and reconstitution of membrane-bound proteins in their native state and for the preparation of lipid vesicles. Its well-defined chemical structure, small uniform micelles and high water solubility make it superior to most other non-ionic detergents for membrane solubilization. The high critical micelle concentration (0.7%) of n-octylglucoside facilitates ready removal from final protein extracts by dialysis or gel filtration. Used in lysis and solubilisation buffers for bacterial lipoprotein preparations, mitochondrial extracts or for determination of protein content by BCA assay. Can be used in 2D electrophoresis and to improve selectivity of immunoprecipitation of phosphotyrosine modified proteins and to increase the resolution of proteins in 2D gels.