Biological Buffers

Most chemical reactions are severely influenced by pH changes of the solution. Biological buffers allow the researcher to maintain control of the solution’s acidity by capturing or releasing protons. The pH value is kept constant even after addition of acids of bases. This is of special importance with regards to biochemical reactions, as the charge of proteins and nucleic acids is strongly correlated to the local pH. Biological solutions like blood or saliva are therefore buffered, mostly by phosphate- or carbonate buffer systems. In molecular biology and biochemistry, buffering is achieved by addition of so-called “good buffers” or PBS.

Most chemical reactions are severely influenced by pH changes of the solution. Biological buffers allow the researcher to maintain control of the solution’s acidity by capturing or releasing... read more »
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Biological Buffers

Most chemical reactions are severely influenced by pH changes of the solution. Biological buffers allow the researcher to maintain control of the solution’s acidity by capturing or releasing protons. The pH value is kept constant even after addition of acids of bases. This is of special importance with regards to biochemical reactions, as the charge of proteins and nucleic acids is strongly correlated to the local pH. Biological solutions like blood or saliva are therefore buffered, mostly by phosphate- or carbonate buffer systems. In molecular biology and biochemistry, buffering is achieved by addition of so-called “good buffers” or PBS.

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MOPS Sodium Salt (Morpholinopropanesulfonic acid sodium salt)
MOPS Sodium Salt (Morpholinopropanesulfonic acid sodium...

Item number: M4615.500

MOPS Sodium Salt is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good's buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm,...
CAS 71119-22-7
MW: 231.20 D
From 119.00€ *
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HEPES Free Acid (N-[2-Hydroxyethyl] piperazine-N'- [2-ethanesulfonic acid])
HEPES Free Acid (N-[2-Hydroxyethyl] piperazine-N'-...

Item number: H2010.100

HEPES is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good's buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm, chemically...
CAS 7365-45-9
MW: 238.31 D
From 127.00€ *
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MOPSO Free Acid (3-[Morpholino]-2- hydroxypropanesulfonic acid)
MOPSO Free Acid (3-[Morpholino]-2- hydroxypropanesulfonic...

Item number: M4620.500

MOPSO Free Acid is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good's buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm,...
CAS 68399-77-9
MW: 225.30 D
From 133.00€ *
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TRIS with EDTA [TE Buffer ] (100x) Endotoxin-free (sterile)
TRIS with EDTA [TE Buffer ] (100x) Endotoxin-free (sterile)

Item number: IAX-900-005-3010

Solubilisation and storage buffer for DNA, diluting 1:100 with sterile endotoxin-free water (IAX-900-002).
Keywords: TE Buffer, Tris(hydroxymethyl)aminomethane with Ethylendiamintetraacetate
From 65.00€ *
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HEPES Buffer (50mM) with NaCl [Sodium Chloride] (150mM) Endotoxin-free (sterile)
HEPES Buffer (50mM) with NaCl [Sodium Chloride] (150mM)...

Item number: IAX-900-009-3010

HEPES Buffer (50mM) with NaCl [Sodium Chloride] (150mM) Endotoxin-free (sterile) is a balanced salt solution used for a wide range of applications (cell culture, washing cells, transporting cells or tissue).
Keywords: 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid Buffer with Sodium Chloride (150mM)
Application: Balanced salt solution
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TES, Sodium Salt (N-[tris-hydroxymethyl] methyl-2-aminoethanesulfonic acid sodium salt)
TES, Sodium Salt (N-[tris-hydroxymethyl]...

Item number: T2905.250

TES Sodium Salt is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good's buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm,...
CAS 70331-82-7
MW: 251.20 D
897.00€ *
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PIPES, Sodium Salt (Piperazine-N,N'-bis(2-ethane sulfonic acid) sodium salt)
PIPES, Sodium Salt (Piperazine-N,N'-bis(2-ethane sulfonic...

Item number: P4205.1

PIPES Sodium is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good's buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm,...
CAS 10010-67-0
MW: 324.30 D
From 270.00€ *
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TAPS, Sodium Salt (3-[N-tris-(hydroxymethyl) methylamino]-propanesulfonic acid Sodium Salt)
TAPS, Sodium Salt (3-[N-tris-(hydroxymethyl)...

Item number: T1005.1

TAPS Sodium is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good's buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm,...
CAS 91000-53-2
MW: 243.30 D
From 190.00€ *
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PBS with EDTA Endotoxin-free (sterile)
PBS with EDTA Endotoxin-free (sterile)

Item number: IAX-900-004-3010

PBS is isotonic at physiological pH and non-toxic to most cells. PBS has many uses which include substance dilution and cell container rinsing. PBS with EDTA is used to detach and dissociate clumped cells in cell culture applications.
Keywords: Phosphate Buffer Saline with EDTA Endotoxin-free (sterile)
From 50.00€ *
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TES Free Acid (N-[tris-hydroxymethyl]methyl-2-aminoethanesulfonic acid)
TES Free Acid...

Item number: T2900.100

TES is a biological buffer (zwitterionic) designed by Good, et al. Typically referred to as Good's Buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240-700nm, chemically stable,...
CAS 7365-44-8
MW: 229.25 D
From 247.00€ *
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NP-40 Cell Lysis Buffer (Mild)
NP-40 Cell Lysis Buffer (Mild)

Item number: BPS-82124-1

The NP-40 Cell Lysis Buffer is intended for the lysis of mammalian adherent and suspension-cultured cells. This buffer is suitable for extraction of cytoplasmic proteins in mild, non-denaturing conditions.
From 61.00€ *
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Modified NP-40 Cell Lysis Buffer (Mild)
Modified NP-40 Cell Lysis Buffer (Mild)

Item number: BPS-82125-1

The Modified NP-40 Cell Lysis Buffer is intended for the lysis of mammalian adherent and suspension-cultured cells. This buffer is stronger than the standard NP-40 Cell Lysis Buffer.
From 83.00€ *
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