Biological Buffers

Most chemical reactions are severely influenced by pH changes of the solution. Biological buffers allow the researcher to maintain control of the solution’s acidity by capturing or releasing protons. The pH value is kept constant even after addition of acids of bases. This is of special importance with regards to biochemical reactions, as the charge of proteins and nucleic acids is strongly correlated to the local pH. Biological solutions like blood or saliva are therefore buffered, mostly by phosphate- or carbonate buffer systems. In molecular biology and biochemistry, buffering is achieved by addition of so-called “good buffers” or PBS.

Most chemical reactions are severely influenced by pH changes of the solution. Biological buffers allow the researcher to maintain control of the solution’s acidity by capturing or releasing... read more »
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Biological Buffers

Most chemical reactions are severely influenced by pH changes of the solution. Biological buffers allow the researcher to maintain control of the solution’s acidity by capturing or releasing protons. The pH value is kept constant even after addition of acids of bases. This is of special importance with regards to biochemical reactions, as the charge of proteins and nucleic acids is strongly correlated to the local pH. Biological solutions like blood or saliva are therefore buffered, mostly by phosphate- or carbonate buffer systems. In molecular biology and biochemistry, buffering is achieved by addition of so-called “good buffers” or PBS.

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Tris Buffer Saline with Tween, 10X (TBST)
Tris Buffer Saline with Tween, 10X (TBST)

Item number: ICT-AR-6564-02

This buffer is supplied as 10X solution. It is used as 1X in all western blotting, ELISA and Chemiluminescence assays as a washing buffer, making blocking solution, diluting HRP-secondary and primary antibody etc.
From 88.00€ *
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Phosphate Buffer, 10X
Phosphate Buffer, 10X

Item number: ICT-AR-6565-02

This buffer is supplied as 10X solution. It is used as 1X in all western blotting, ELISA and Chemiluminescence assays as a washing buffer, making blocking solution, diluting HRP-secondary and primary antibody etc.
From 88.00€ *
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Citrate Buffer, 10X
Citrate Buffer, 10X

Item number: ICT-AR-6544-02

This buffer is intended for heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections prior to application of antibodies.
From 88.00€ *
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Immuno Automation Buffer(TM) 10X
Immuno Automation Buffer(TM) 10X

Item number: ICT-AR-6561-02

This buffer is supplied as 10X solution. It is used as 1X in all immunoassays (Immunofluorescence, IHC, ELISA, WB etc), as a washing buffer and can be used as dilution buffer for antibodies, however it does not contain any carrier proteins.
From 88.00€ *
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Antibody Dilution Buffer with BSA (Immunoglobulin free)
Antibody Dilution Buffer with BSA (Immunoglobulin free)

Item number: ICT-AR-6563-02

This antibody dilution buffer is ready-to-use for dilution of antibodies in all immunoassays (Immunofluorescence, IHC, ELISA, and WB).
From 88.00€ *
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Peroxidase Stabilizing Buffer
Peroxidase Stabilizing Buffer

Item number: ICT-AR-6567-02

This horseradish peroxidase (HRP) stabilizing buffer is ready-to-use for peroxidase conjugates and as negative control in immunoassays.
From 138.00€ *
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Tris Hydrochloride (Tris(hydroxymethyl) aminomethane_HCl,_2-Amino-2- (hydroxymethyl)-1, 3-propanedio
Tris Hydrochloride (Tris(hydroxymethyl)...

Item number: T8650.1

Buffering agent in incubation mixtures. It has also been used as a component of lysis and TE (Tris-EDTA) buffer. One of the biological buffers. Synonyms: Tris(hydroxymethyl)aminomethane HCl, 2-Amino-2-(hydroxymethyl)-1,3-propanediol HCl, Tromethamine HCl, CAS No: C4H11NO3HCl, Molecular Formula: C4H11NO3HCl,...
CAS 1185-53-1
MW: 157.60 D
From 199.00€ *
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1X PBS (2L) Buffer Powder Packets (box of 50 packets)
1X PBS (2L) Buffer Powder Packets (box of 50 packets)

Item number: BOT-22033

PBS (phosphate-buffered saline) buffer powder packet for preparing 2 L of 1X PBS buffer. Box includes 50 packets.
Keywords: PBS, phosphate-buffered saline, buffer
171.00€ *
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1X TBE (1L) Buffer Powder Packets (box of 50 packets)
1X TBE (1L) Buffer Powder Packets (box of 50 packets)

Item number: BOT-22032

TBE (Tris-borate-EDTA) buffer powder packet for preparing 1 L of 1X TBE buffer for nucleic acid gel electrophoresis. Box includes 50 packets.
Keywords: TBE, gel electrophoresis, nucleic acids, agarose gels
213.00€ *
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TES, Sodium Salt (N-[tris-hydroxymethyl] methyl-2-aminoethanesulfonic acid sodium salt)
TES, Sodium Salt (N-[tris-hydroxymethyl]...

Item number: T2905.1

TES Sodium Salt is a biological buffer (zwitterionic) designed by Good, et al., and typically referred to as Good's buffer useful in cell culture media formulations. Selection of biological buffer systems should include the following criteria: exclusion by biological membranes, low absorption between 240 and 700nm,...
CAS 70331-82-7
MW: 251.20 D
From 447.00€ *
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ddWater Endotoxin-free (sterile)
ddWater Endotoxin-free (sterile)

Item number: IAX-900-002-LD15

Endotoxin free diluent buffer used for the dilution of TLRpure LPS, MPLA and Lipid A and solubilisation of endotoxin-free reagents (e.g. ODNs).
Keywords: H2O, double-distilled Water
CAS 7732-18-5
MW: 18 D
From 34.00€ *
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PBS Endotoxin-free (sterile)
PBS Endotoxin-free (sterile)

Item number: IAX-900-001-3010

PBS Endotoxin-free (sterile) is a balanced salt solution used for a wide range of applications (cell culture, washing cells, transporting cells or tissue, diluting cells for counting, and preparing other laboratory reagents). This PBS product is without calcium and magnesium for the purpose of rinsing cells before...
Keywords: Phosphate Buffer Saline Endotoxin-free (sterile)
From 34.00€ *
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