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Vinyl-L-NIO is a potent, selective inhibitor of nNOS. The Ki values for inhibition of nNOS, eNOS, and iNOS by vinyl-L-NIO are 100 nM, 12 and 60 µM, respectively, as determined using initial rate measurements. In the presence of NADPH and O2, vinyl-L-NIO irreversibly inactivates nNOS with a kinact of 0.078 min-1 and a Ki value of 90 nM. Vinyl-L-NIO does not inactivate iNOS. eNOS requires 20-fold higher concentrations of vinyl-L-NIO to obtain 75% the rate of inactivation seen with nNOS. Formal Name: N5-(1-imino-3-butenyl)-L-ornithine, monohydrochloride. CAS Number: 728944-69-2. Molecular Formula: C9H17N3O2 . HCl. Formula Weight: 235.7. Purity: >95%. Formulation: A film. Solubility: DMF: 60 mg/ml, DMSO: 50 mg/ml, Ethanol: 30 mg/ml, PBS (pH 7.2): 10 mg/ml. SMILES: C=CCC(NCCC[C@H](N)C(O)=O)=N.Cl. InChi Code: InChI=1S/C9H17N3O2.ClH/c1-2-4-8(11)12-6-3-5-7(10)9(13)14,/h2,7H,1,3-6,10H2,(H2,11,12)(H,13,14),1H. InChi Key: DIWIMDZKSYTKCZ-UHFFFAOYSA-N.