Trolox

White to off-white or pale yellow solid. Soluble in DMSO (20mg/ml), 100% ethanol (20mg/ml) or methanol (5mg/ml). Slightly soluble in water (~1mg/ml) and PBS (3mg/ml). Cell permeable and water soluble derivative of vitamin E, Potent antioxidant. Efficiently prevents formation of different reactive oxygen species, such as singlet oxygen (1O2), superoxide anion (O2-) or hydrogen peroxide (H2O2), Commonly used as a standard or positive control in different antioxidant assays, such as ABTS decolorization and FRAP assays, measuring Trolox equivalent antioxidant capacity (TEAC). TEAC assays are used to measure antioxidant capacity of foods, beverages and supplements, Used in biological or biochemical applications to reduce oxidative stress or damage, such as inhibiting peroxynitrite mediated tyrosine nitration and mitochondrial proton permeability, Cytoprotective with low cytotoxicity for different cell lines. Shown to have anticancer activity and to be effective as adjunctive agent in cancer treatments, Used to reduce fluorophore bleaching as well as the fluorophore blinking during the observation of fluorescent labels, Freezing extender supplement in cryopreservation media to improve the quality of cryopreserved human sperm.