Tosyllysine Chloromethyl Ketone (hydrochloride)

Tosyllysine chloromethylketone (TLCK) is an active site-directed agent that inhibits serine proteinases with trypsin-like activity. TLCK may also act non-selectively with thiol groups and thereby inhibit cysteine proteinases and other enzymes. To prevent proteolytic degradation, TLCK may be used in protein purification protocols. TLCK selectively inactiviates clostripain obtained from C. histolyticum.Formal Name: N-[5-amino-1-(2-chloroacetyl)pentyl]-4-methyl-benzenesulfonamide, monohydrochloride. CAS Number: 4272-74-6. Synonyms: 1-Chloro-3-Toxylamide-7-Amino-L-2-Heptanone, TLCK. Molecular Formula: C14H21ClN2O3S . HCl. Formula Weight: 369.3. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 20 mg/ml, DMSO: 5 mg/ml, PBS (pH 7.2): 5 mg/ml. lambdamax: 229 nm. SMILES: CC1=CC=C(S(N[C@@H](CCCCN)C(CCl)=O)(=O)=O)C=C1.Cl. InChi Code: InChI=1S/C14H21ClN2O3S.ClH/c1-11-5-7-12(8-6-11)21(19,20)17-13(14(18)10-15)4-2-3-9-16,/h5-8,13,17H,2-4,9-10,16H2,1H3,1H/t13-,/m0./s1. InChi Key: YFCUZWYIPBUQBD-ZOWNYOTGSA-N.