Tetrazolium Blue Chloride

Tetrazolium Blue Chloride (Nitro Blue Tetrazolium, NBT) is a redox-sensitive dye widely used as a chromogenic reagent for detecting enzymatic activity, reactive oxygen species (ROS), and cellular respiration. Upon reduction by dehydrogenases or superoxide radicals, it forms an insoluble blue formazan precipitate, enabling visual or spectrophotometric quantification of metabolic activity. NBT is commonly used as a colorimetric reagent and applied in microbiology, histochemistry, and plant and animal cell research to assess oxidative burst, mitochondrial function, and electron transport chain activity. Spectral Data: lambdaabs ~540 nm, with a wide wavelength range of 480-600 nm.