Sulfo-Cyanine3 UTP

Sulfo-Cyanine3 UTP can be used as a substitute for UTP as a substrate for T7 RNA polymerase, enabling the production of labeled probes via in vitro transcription. It has excitation and emission maxima at 550 nm and 570 nm, respectively. The Sulfo-Cyanine3 dye imparts fluorescent properties to the UTP molecule, allowing Sulfo-Cyanine3 UTP to be incorporated into RNA molecules through RNA polymerase-catalyzed transcription reactions. This enables researchers to track RNA distribution, localization, and dynamics. mRNA labeled with Sulfo-Cyanine3 UTP is easily visible under UV light and eliminates the need for additional staining after gel electrophoresis. Additionally, Sulfo-Cyanine3 UTP is suitable for single-molecule fluorescence resonance energy transfer (FRET) measurements, facilitating real-time monitoring of the co-transcriptional folding of RNA molecules [4]. This product is supplied as a 10 mM aqueous solution. Chemical Properties Storage Upon receipt, store at -20°C in the dark. Protect from light and moisture. When stored as indicated, Sulfo-Cyanine3 UTP is stable for 1 year. This product is for Research Use Only· Not for Human or Veterinary or Therapeutic Use CAS number NA Formula C42H54N5O22P3S2 Molecular Weight 1137.95 Concentration 10.0 mM Solvent 10 mM Tris-HCl, pH 7.6, 1.0 mM EDTA HPLC Purity >95% lambdaabs (nm) 550 lambdafl (nm) 568 epsilonmax (M-1 cm-1) 1.5×105