Sarsasapogenin

Sarsasapogenin is a steroid sapogenin originally isolated from Smilax that has antiproliferative, pro-apoptotic, anti-inflammatory, neuroprotective, and antidepressant-like properties. Sarsasapogenin inhibits proliferation of HeLa cervical, MCF-7 breast, HepG2 liver, A549 lung, A375.S2 melanoma, and HT-1080 fibrosarcoma cells (IC50s = 31.36-48.79 µM) and dose-dependently induces apoptosis of HeLa cells. It also protects human SH-SY5Y neuroblastoma cells from hydrogen peroxide-induced death with a neuroprotective ratio of 27.3% when used at a concentration of 100 µM. In vivo, sarsasapogenin (10 mg/kg) reduces 2,3,4-trinitrobenzene sulfonic acid-induced colon shortening, myeloperoxidase activity, NF-kappaB activation, and IL-1beta, TNF-alpha, and IL-6 levels in mice. It also increases the percentage of correct responses in the Y-maze test in aged rats when administered at a dose of 3.6 mg/kg per day. Sarsasapogenin (12.5 mg/kg per day) reduces the amount of time mice spend immobile in the forced swim test, indicating antidepressant-like behavior.Formal Name: (3beta,5beta,25S)-spirostan-3-ol. CAS Number: 126-19-2. Synonyms: NSC 1615. Molecular Formula: C27H44O3. Formula Weight: 416.6. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 2 mg/ml, DMSO: 0.2 mg/ml, Ethanol: 2 mg/ml, Ethanol:PBS (pH 7.2)(1:2): 0.3 mg/ml. SMILES: C[C@@H]1[C@]2(OC[C@@H](C)CC2)O[C@@]3([H])C[C@@]4([H])[C@]5([H])CC[C@]6([H])C[C@@H](O)CC[C@]6(C)[C@@]5([H])CC[C@]4(C)[C@]31[H]. InChi Code: InChI=1S/C27H44O3/c1-16-7-12-27(29-15-16)17(2)24-23(30-27)14-22-20-6-5-18-13-19(28)8-10-25(18,3)21(20)9-11-26(22,24)4/h16-24,28H,5-15H2,1-4H3/t16-,17-,18+,19-,20+,21-,22-,23-,24-,25-,26-,27+/m0/s1. InChi Key: GMBQZIIUCVWOCD-WWASVFFGSA-N.