Rbin-1

Rbin-1 is a triazinoindole inhibitor of midasin (mdn1), an essential protein for eukaryotic ribosome biogenesis. It inhibits the growth of fission yeast strains in vitro (GI50 = 136 nM) and inhibits mdn1 ATPase activity by approximately 40% when used at a concentration of 1 µM. Rbin-1 inhibits pre-rRNA processing and nuclear export of the pre-60S ribosome subunit in mdn1-ts, but not mdn1-F1093L mutant, S. pombe cells. It also decreases ribosome assembly protein 4 (Rsa4) levels in Rix1 particles, a ribosome assembly intermediate, Rix1-bound Ytm1 levels, and Rix7 and Ppp1 levels in the nucleolar pre-60S particle Nsa1 in mdn1-ts fission yeast cells.Formal Name: 3-[(2-methyl-2-propen-1-yl)thio]-5H-1,2,4-triazino[5,6-b]indole. CAS Number: 328023-11-6. Synonyms: Ribozinoindole-1. Molecular Formula: C13H12N4S. Formula Weight: 256.3. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 5 mg/ml, DMSO: 25 mg/ml. lambdamax: 224, 266, 346 nm. SMILES: C=C(C)CSC(N=N1)=NC2=C1C3=CC=CC=C3N2. InChi Code: InChI=1S/C13H12N4S/c1-8(2)7-18-13-15-12-11(16-17-13)9-5-3-4-6-10(9)14-12/h3-6H,1,7H2,2H3,(H,14,15,17). InChi Key: LPCWHNSRTRBKBO-UHFFFAOYSA-N.