Oregon Green(TM) 488

Oregon Green(TM) 488 is a fluorinated fluorescein dye. It exists as a tautomer in aqueous solutions. Oregon Green(TM) 488 has minimal pH dependence at physiological pH but it can be used as an indicator for intracellular pH due to its low pKa of 4.8. It is less susceptible to photobleaching than fluorescein. Oregon Green(TM) 488 has been conjugated to antibodies and proteins, as well as DNA, for use in labeling and detection. It displays excitation/emission maxima of 490/514 nm, respectively.Formal Name: 2',7'-difluoro-3',6'-dihydroxy-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one. CAS Number: 195136-58-4. Synonyms: OG 488, 2',7'-Difluorofluorescein. Molecular Formula: C20H10F2O5. Formula Weight: 368.3. Purity: >95%. Emission: 514 nm. Excitation: 490 nm. Formulation: (Request formulation change), A solid. Solubility: Chloroform: Soluble, DMSO: Soluble. SMILES: O=C(O1)C2=CC=CC=C2C31C4=CC(F)=C(O)C=C4OC5=CC(O)=C(F)C=C53. InChi Code: InChI=1S/C20H10F2O5/c21-13-5-11-17(7-15(13)23)26-18-8-16(24)14(22)6-12(18)20(11)10-4-2-1-3-9(10)19(25)27-20/h1-8,23-24H. InChi Key: FJXJIUHGLVUXQP-UHFFFAOYSA-N.