Naphthofluorescein

Furin is a proprotein convertase, converting precursor proteins to functional proteins within the Golgi/trans-Golgi secretory pathway. Naphthofluorescein is a cell-permeable inhibitor of furin (IC50 = 12 µM). It inhibits furin-mediated cleavage of the pro-form of membrane type-1 matrix metalloproteinase (MT1-MMP), resulting in decreased levels of active MT1-MMP. This, in turn, suppresses MMP-2 activation and reduces cell motility in CHO cells expressing proMT1-MMP. Napthofluorescein significantly inhibits the invasion of Matrigel by the human fibrosarcoma cell line, HT1080. The effects of this furin inhibitor on other furin-mediated processes, in vivo or in vitro, remain to be determined.Formal Name: 3,11-dihydroxy-spiro[7H-dibenzo[c,h]xanthene-7,1'(3'H)-isobenzofuran]-3'-one. CAS Number: 61419-02-1. Synonyms: CCG 8295. Molecular Formula: C28H16O5. Formula Weight: 432.4. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 10 mg/ml, DMF:PBS (pH 7) (1:10): 0.09 mg/ml, DMSO: 3 mg/ml, Ethanol: 2 mg/ml. lambdamax: 233, 255 nm. SMILES: O=C(C1=C2C=CC=C1)OC32C4=CC=C5C(C=CC(O)=C5)=C4OC6=C3C=CC7=C6C=CC(O)=C7. InChi Code: InChI=1S/C28H16O5/c29-17-7-9-19-15(13-17)5-11-23-25(19)32-26-20-10-8-18(30)14-16(20)6-12-24(26)28(23)22-4-2-1-3-21(22)27(31)33-28/h1-14,29-30H. InChi Key: IXQIUDNVFVTQLJ-UHFFFAOYSA-N.