Mitotane

Mitotane (o,p'DDD) is an adrenocorticolytic agent primarily used in research related to adrenal gland disorders, especially adrenocortical carcinoma (ACC). Mitotane is an inhibitor of steroidogenesis and mitochondrial complex IV, also known as cytochrome c oxidase. It selectively targets adrenal cortex cells by disrupting mitochondrial function and steroidogenesis, leading to cytotoxic and endocrine-suppressive effects. Mitotane is a selective inhibitor of sterol-Oacyl-transferase 1 (SOAT1), also known as ACAT1, (acyl-coenzyme A cholesterol acyltransferase). Mitotane inhibits enzymes involved in cortisol synthesis, such as CYP11A1 and CYP11B1, and induces adrenal atrophy. Mitotane acts through mitochondrial toxicity (induction of apoptosis), inhibition of cell proliferation in ACC, inhibition of steroidogenic enzymes, and selective adrenal cytotoxicity, making it effective in treating adrenocortical carcinoma and Cushing's syndrome. Compound can be used as analytical reference material.