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L-NIO is a non-selective inhibitor of all NOS isoforms. The Ki values for nNOS (rat), eNOS (bovine), and iNOS (mouse) are 1.7, 3.9, and 3.9 µM, respectively. L-NIO inhibits endothelial-dependent relaxation induced by acetylcholine in rat aortic rings and hypotensive responses to acetylcholine and bradykinin. Formal Name: N5-(1-iminoethyl)-L-ornithine, dihydrochloride. CAS Number: 36889-13-1. Molecular Formula: C7H15N3O2 . 2HCl. Formula Weight: 246.1. Purity: >95%. Formulation: A crystalline solid. Solubility: DMF: 20 mg/ml, DMSO: 20 mg/ml, Ethanol: 20 mg/ml, PBS (pH 7.2): 10 mg/ml, Water: 100 mg/ml. SMILES: CC(=N)NCCC[C@H](N)C(=O)O. InChi Code: InChI=1S/C7H15N3O2/c1-5(8)10-4-2-3-6(9)7(11)12/h6H,2-4,9H2,1H3,(H2,8,10)(H,11,12). InChi Key: UYZFAUAYFLEHRC-UHFFFAOYSA-N.