KLH45

KLH45 is an inhibitor of the phospholipase DDHD domain containing 2 (DDHD2, IC50 = 1.3 nM). It is selective for DDHD2 over greater than 50 serine hydrolases, including DDHD1 and Sec23ip, but does inhibit alpha/beta-hydrolase domain-containing protein 6 (ABHD6). It inhibits DDHD2 and ABHD6 in Neuro2A cells when used at a concentration of less than 10 nM and inhibits DDHD2 by greater than 95% at a concentration of 25 nM. KLH45 (2 µM) prevents increases in triacylglycerol (TAG) hydrolase activity in HEK293T cell lysates expressing recombinant DDHD2 and incubated with a radiolabeled TAG substrate. It decreases TAG levels in the CNS of mice when administered at a dose of 20 mg/kg twice per day for four days. KLH45 (2 µM) also reverses DDHD2-induced lipid droplet reduction in COS-7 cells expressing recombinant DDHD2 and loaded with oleic acid.Formal Name: N-cyclohexyl-N-(2-phenylethyl)-4-[4-(trifluoromethoxy)phenyl]-2H-1,2,3-triazole-2-carboxamide. CAS Number: 1632236-44-2. Molecular Formula: C24H25F3N4O2. Formula Weight: 458.5. Purity: >98%. Formulation: (Request formulation change), A solution in ethanol. Solubility: DMSO: 30 mg/ml, Ethanol: 30 mg/ml. lambdamax: 206, 262 nm. SMILES: O=C(N(C1CCCCC1)CCC2=CC=CC=C2)N3N=C(C4=CC=C(OC(F)(F)F)C=C4)C=N3. InChi Code: InChI=1S/C24H25F3N4O2/c25-24(26,27)33-21-13-11-19(12-14-21)22-17-28-31(29-22)23(32)30(20-9-5-2-6-10-20)16-15-18-7-3-1-4-8-18/h1,3-4,7-8,11-14,17,20H,2,5-6,9-10,15-16H2. InChi Key: FYOYNRLSBYWAHL-UHFFFAOYSA-N.