Indo-1 AM

Indo-1 AM is a cell-permeant acetoxymethyl ester of the ratiometric fluorescent calcium indicator indo-1 (Cay-20418, Cay-20419). As indo-1 AM enters cells, it is hydrolyzed by intracellular esterases to produce Indo-1. Indo-1 is ideal for analyses using flow cytometry, as it uses a single excitation source, typically 349-364 nm light from an argon-ion laser. The emission maximum shifts from 475-485 nm without calcium to 400-410 nm when indo-1 binds calcium. Indo-1 is prone to photobleaching, which limits its usefulness in methods involving microscopy.Formal Name: 2-[4-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-3-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-1H-Indole-6-carboxylic acid, (acetyloxy)methyl ester. CAS Number: 112926-02-0. Synonyms: Indo-1 Acetoxymethyl ester. Molecular Formula: C47H51N3O22. Formula Weight: 1009.9. Purity: >90%. Emission: 475-485 nm (no Ca2+), 400-410 nm (Ca2+-bound). Excitation: 349-364 nm. Formulation: (Request formulation change), A solid. Solubility: DMSO: Soluble. SMILES: O=C(OCOC(C)=O)CN(CC(OCOC(C)=O)=O)C1=CC=C(C2=CC3=C(C=C(C(OCOC(C)=O)=O)C=C3)N2)C=C1OCCOC4=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=CC(C)=C4. InChi Code: InChI=1S/C47H51N3O22/c1-28-7-11-39(49(19-43(56)68-23-63-29(2)51)20-44(57)69-24-64-30(3)52)41(15-28)61-13-14-62-42-18-35(37-16-34-8-9-36(17-38(34)48-37)47(60)72-27-67-33(6)55)10-12-40(42)50(21-45(58)70-25-65-31(4)53)22-46(59)71-26-66-32(5)54/h7-12,15-. InChi Key: CAWBRCOBJNWRLK-UHFFFAOYSA-N.