IMP-1710

IMP-1710 is an inhibitor of ubiquitin C-terminal hydrolase L1 (UCH-L1, IC50 = 38 nM in a fluorescence polarization assay) that contains an alkyne moiety for use in click chemistry reactions. It is selective for UCH-L1 over a panel of 20 deubiquitinating enzymes at 1 µM. IMP-1710 inhibits TGF-beta1-induced fibroblast-to-myofibroblast transition (FMT) in primary lung fibroblasts isolated from patients with idiopathic pulmonary fibrosis (IC50 = 740 nM). It has been used for labeling UCH-L1 in cell-based assays, followed by click reactions with azide-modified TAMRA and biotin capture reagents.Formal Name: (2S)-2-[[4-(5-ethynyl-1H-pyrrolo[2,3-b]pyridin-3-yl)-2,3-dihydro-1H-indol-1-yl]carbonyl]-1-pyrrolidinecarbonitrile. CAS Number: 2383117-96-0. Synonyms: Click Tag(TM) IMP-1710. Molecular Formula: C23H19N5O. Formula Weight: 381.4. Purity: >98%. Formulation: (Request formulation change), A solution in ethanol. Solubility: Chloroform: 10 mg/ml, Ethanol: 5 mg/ml. lambdamax: 249 nm. SMILES: O=C([C@H]1N(C#N)CCC1)N2C3=CC=CC(C(C4=C5)=CNC4=NC=C5C#C)=C3CC2. InChi Code: InChI=1S/C23H19N5O/c1-2-15-11-18-19(13-26-22(18)25-12-15)16-5-3-6-20-17(16)8-10-28(20)23(29)21-7-4-9-27(21)14-24/h1,3,5-6,11-13,21H,4,7-10H2,(H,25,26)/t21-/m0/s1. InChi Key: FYMVGXGSNIVSKY-NRFANRHFSA-N.