Green CMFDA

Green CMFDA is a cell-permeable fluorescent probe that freely enters living cells, where it is transformed into a cell-impermeable, soluble, fluorescent compound. The resulting molecule does not affect cell viability or proliferation and stably fluoresces for at least 72 hours, allowing for its use in cell tracking. It is also transferred to daughter cells. Green CMFDA is brightly fluorescent with standard filters (ex/em max = 492/517 nm) and is often used with additional fluorescent dyes and proteins.Formal Name: 3',6'-bis(acetyloxy)-5-(chloromethyl)-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one. CAS Number: 136832-63-8. Synonyms: 5-Chloromethylfluorescein diacetate. Molecular Formula: C25H17ClO7. Formula Weight: 464.9. Purity: >95%. Emission: 517 nm. Excitation: 492 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: 0.1 M Na2CO3: 5 mg/mL, DMF: 25 mg/mL, DMSO: 30 mg/mL, Ethanol: 25 mg/mL, PBS (pH 7.2): 0.2 mg/ml. lambdamax: 220, 291 nm. SMILES: O=C(O1)C2=C(C=CC(CCl)=C2)C31C4=CC=C(OC(C)=O)C=C4OC5=C3C=CC(OC(C)=O)=C5. InChi Code: InChI=1S/C25H17ClO7/c1-13(27)30-16-4-7-20-22(10-16)32-23-11-17(31-14(2)28)5-8-21(23)25(20)19-6-3-15(12-26)9-18(19)24(29)33-25/h3-11H,12H2,1-2H3. InChi Key: IPJDHSYCSQAODE-UHFFFAOYSA-N.