Golgicide A

ADP-ribosylation factor 1 (Arf1), when activated by guanine nucleotide exchange factors (ArfGEFs), plays a key role in regulating secretory traffic and membrane transport within the Golgi of eukaryotic cells. Golgicide A is a reversible inhibitor of the brefeldin A (Cay-11861)-resistant, cis-Golgi ArfGEF, GBF1 (IC50 = 3.3 µM). Inhibition of GBF1 via golgicide A can result in rapid dissociation of COPI vesicle coat protein from Golgi membranes and disassembly of the Golgi and trans-Golgi network. Golgicide A does not affect the association of the adaptor protein, AP-1, or the Arf1-binding protein, GCA3, with the trans-Golgi network nor does it interfere with the activity of the ArfGEFs, BIG1 or 2. Golgicide A can arrest the secretion of soluble and membrane-associated proteins at the ER-Golgi intermediate compartment, as well as prevent endocytic transport of Shiga toxin to the trans-Golgi network.Formal Name: 6,8-difluoro-3a,4,5,9b-tetrahydro-4-(3-pyridinyl)-3H-cyclopenta[c]quinoline. CAS Number: 1005036-73-6. Synonyms: GCA. Molecular Formula: C17H14F2N2. Formula Weight: 284.3. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMF:PBS (pH 7.2) (1:8): 0.1mg/ml, DMSO: 10 mg/ml, Ethanol: 1 mg/ml. lambdamax: 241, 294 nm. SMILES: FC1=CC2=C(C(F)=C1)NC(C3=CN=CC=C3)C4C2C=CC4. InChi Code: InChI=1S/C17H14F2N2/c18-11-7-14-12-4-1-5-13(12)16(10-3-2-6-20-9-10)21-17(14)15(19)8-11/h1-4,6-9,12-13,16,21H,5H2. InChi Key: NJZHEQOUHLZCOX-UHFFFAOYSA-N.