Fluorescein dicaproate

Fluorescein dicaproate is a non-fluorescent hydrophobic fluorescein derivative that can pass through the cell membrane whereupon intracellular esterases hydrolyze the dicaproate group producing the highly fluorescent product fluorescein. The fluorescein molecules accumulate in cells. Fluorescein dicaproate is a fluorogenic substrate for C6 esterase that generates green fluorescence upon esterase cleavage. Spectral Data: lambdaEx 497nM, &lamda,Em 516nm. Extinction coefficient: 80000 cm-1 M-1. Product References: (1) B. Rotman & B.W. Papermaster, PNAS 55, 134 (1966) , (2) F.-Y. Ge, et al., Dyes Pigm. 72, 322 (2007) Appearance: Faint yellow powder. Solubility: Soluble in DMSO or chloroform. Identity: Determined by 1H-NMR. InChi Key: DHSNCRHSIFNRJY-UHFFFAOYSA-N Smiles: O=C(O1)C2=C(C=CC=C2)C31C4=C(C=C(OC(CCCCC)=O)C=C4)OC5=CC(OC(CCCCC)=O)=CC=C53