Fluorescein diacetate

Fluorescein diacetate
Item number Size Datasheet Manual SDS Delivery time Quantity Price
CDX-F0027-G010 10 g - -

3 - 9 business days*

99.00€
CDX-F0027-G025 25 g - -

3 - 9 business days*

199.00€
CDX-F0027-G100 100 g - -

3 - 9 business days*

604.00€
 
White to off-white powder. Soluble in DMSO, ethanol, water, chloroform or acetone (25mg/ml).... more
Product information "Fluorescein diacetate"
White to off-white powder. Soluble in DMSO, ethanol, water, chloroform or acetone (25mg/ml). Chemical. CAS: 596-09-8. Formula: C24H16O7. MW: 416.38. Synthetic. FDA is a non-fluorescent hydrophobic fluorescein derivative that can pass through the cell membrane whereupon intracellular esterases hydrolyze the diacetate group producing the highly fluorescent product fluorescein. The fluorescein molecules accumulate in cells that possess intact membranes so the green fluorescence can be used as a marker of cell viability. Cells that do not possess an intact cell membrane or an active metabolism may not accumulate the fluorescent product and therefore do not exhibit green fluorescence. FDA may be used in combination with PI staining as the non-viable cells take up the PI and stain dead cells red whereas viable cells do not take up the PI and should only stain green. This 2-color separation of non-viable and viable cells may provide a more accurate quantitation of cell viability than single color analysis. FDA is used in studies on intracellular interactions and membrane permeability, for flow cytometry and as esterase substrate. Spectral data: lambdaex=494nm, lambdaem=521nm.
Keywords: FDA, 3,6-Diacetoxyfluoran, Di-O-Acetylfluorescein, Cellstain-FDA, NSC 4726, NSC 667259
Supplier: Chemodex
Supplier-Nr: F0027

Properties

Application: Staining
MW: 416,38 D
Formula: C24H16O7
Purity: >95% (NMR)
Format: Solid

Database Information

CAS : 596-09-8| Matching products

Handling & Safety

Storage: -20°C
Shipping: +20°C (International: +20°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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