Fluorescein Biotin

Fluorescein biotin (FITC biotin) is a fluorescent biotin derivative and avidin and streptavidin ligand that contains a FITC fluorophore which displays excitation/emission maxima of 485/535 nm, respectively. FITC fluorescence is quenched upon binding to avidin or streptavidin and decreases in fluorescence intensity have been used to quantify biotin binding stoichiometry.Formal Name: (3aS,4S,6aR)-N-[6-[[5-[[[(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)amino]thioxomethyl]amino]pentyl]amino]-6-oxohexyl]hexahydro-2-oxo-1H-thieno[3,4-d]imidazole-4-pentanamide. CAS Number: 134759-22-1. Synonyms: FITC Biotin. Molecular Formula: C42H50N6O8S2. Formula Weight: 831.0. Purity: >90%. Emission: 535 nm. Excitation: 485 nm. Formulation: (Request formulation change), A solid. Solubility: DMF: Soluble, DMSO: Soluble, Methanol: Soluble, Water: Slightly Soluble. lambdamax: 222 nm. SMILES: S=C(NCCCCCNC(CCCCCNC(CCCC[C@H]1[C@]2([H])[C@](NC(N2)=O)([H])CS1)=O)=O)NC3=CC=C4C(C(OC45C(C=CC(O)=C6)=C6OC7=C5C=CC(O)=C7)=O)=C3. InChi Code: InChI=1S/C42H50N6O8S2/c49-26-13-16-30-33(22-26)55-34-23-27(50)14-17-31(34)42(30)29-15-12-25(21-28(29)39(53)56-42)46-41(57)45-20-8-2-7-19-44-36(51)10-3-1-6-18-43-37(52)11-5-4-9-35-38-32(24-58-35)47-40(54)48-38/h12-17,21-23,32,35,38,49-50H,1-11,18-20,24H2,(H,43,52)(H,44,51)(H2,45,46,57)(H2,47,48,54)/t32-,35-,38-/m0/s1. InChi Key: MFVSXUVAYGNGJQ-INIOENKZSA-N.