eIF2alpha Activator 2

eIF2alpha activator 2 is an activator of eukaryotic translation initiation factor 2alpha kinase 1 (EIF2AK1), also known as heme-regulated inhibitor kinase (HRI). It activates HRI and induces eIF2alpha phosphorylation in a surrogate cell-based reporter assay. eIF2alpha activator 2 selectively inhibits the proliferation of CRL-2813 melanoma cells (IC50 = 0.46 µM) over non-cancerous NIH3T3 fibroblasts (IC50 = 3.4 µM). It also selectively inhibits the proliferation of CRL-2813 cells transiently transfected with non-targeted siRNA (IC50 = 0.66 µM) over those transiently transfected with HRI-targeted siRNA (IC50 = 5 µM). eIF2alpha activator 2 (5 µM) increases mRNA expression and protein levels of the eIF2alpha target DNA damage-inducible transcript 3 (DDIT3), also known as C/EBP homologous protein (CHOP), in CRL-2813 cells.Formal Name: N-[trans-4-[4-(trifluoromethyl)phenoxy]cyclohexyl]-N'-[3-(trifluoromethyl)phenyl]-urea. CAS Number: 1501618-04-7. Synonyms: Eukaryotic Translation Initiation Factor 2 Subunit 1 Activator 2. Molecular Formula: C21H20F6N2O2. Formula Weight: 446.4. Purity: >98%. Formulation: (Request formulation change), A solid. Solubility: Acetonitrile: Soluble. SMILES: FC(F)(F)C(C=C1)=CC=C1O[C@H](CC2)CC[C@@H]2NC(NC3=CC(C(F)(F)F)=CC=C3)=O. InChi Code: InChI=1S/C21H20F6N2O2/c22-20(23,24)13-4-8-17(9-5-13)31-18-10-6-15(7-11-18)28-19(30)29-16-3-1-2-14(12-16)21(25,26)27/h1-5,8-9,12,15,18H,6-7,10-11H2,(H2,28,29,30)/t15-,18-. InChi Key: MFRDVPYKJUYMDN-RZDIXWSQSA-N.