EC330

EC330 is an inhibitor of leukemia inhibitory factor (LIF) activity. It binds to the LIF receptor (LIFR) and is selectively cytotoxic to MCF-7 cells ectopically overexpressing LIF (IC50 = 0.075 µM) over non-LIF-overexpressing MCF-7 cells (IC50 = 0.245 µM). EC330 reduces ectopic LIF expression-induced increases in the migration of MCF-7 and MDA-MB-231 cells when used at concentrations of 5 and 15 nM, respectively. It also induces ferroptosis in 293T and 786-O cells and reduces the levels of glutathione (GSH) and the intracellular activity of glutathione peroxidase (GPX) in vitro. EC330 (1 mg/kg) reduces tumor burden to a greater extent in a mouse xenograft model using MDA-MB-231 cells with ectopic LIF expression than when using MDA-MB-231 cells without ectopic LIF expression.Formal Name: (11beta,17beta)-11-(4-cyclopropylphenyl)-17-(1,1-difluoro-2-propyn-1-yl)-17-hydroxy-estra-4,9-dien-3-one. CAS Number: 2016795-77-8. Molecular Formula: C30H32F2O2. Formula Weight: 462.6. Purity: >98%. Formulation: (Request formulation change), A solid. Solubility: DMF: 10 mg/ml, DMSO: 10 mg/ml, Ethanol: 20 mg/ml. SMILES: C[C@@]12[C@](CC[C@@]2(O)C(F)(F)C#C)([H])[C@@]3([H])C([C@@H](C4=CC=C(C5CC5)C=C4)C1)=C6C(CC3)=CC(CC6)=O. InChi Code: InChI=1S/C30H32F2O2/c1-3-30(31,32)29(34)15-14-26-24-12-10-21-16-22(33)11-13-23(21)27(24)25(17-28(26,29)2)20-8-6-19(7-9-20)18-4-5-18/h1,6-9,16,18,24-26,34H,4-5,10-15,17H2,2H3/t24-,25+,26-,28-,29-/m0/s1. InChi Key: OWPPSPZDYGHPAN-GCNJZUOMSA-N.