Dihydrorhodamine 123

DHR is a cell-permeable fluorogenic probe that is used as an indicator of intracellular peroxynitrite formation. It is oxidized by peroxynitrite to the highly fluorescent product rhodamine in vitro. Neither nitric oxide, superoxide, nor hydrogen peroxide alone appear to oxidize DHR. Formation of rhodamine can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 500 and 536 nm, respectively, or by absorbance spectroscopy at 500 nm (epsilon = 78,800 M-1cm-1). DHR has been used to investigate reactive oxygen intermediates produced by endothelial cells, eosinophils, and reactive microglia.Formal Name: 2-(3,6-diamino-9H-xanthen-9-yl)-benzoic acid, methyl ester. CAS Number: 109244-58-8. Synonyms: DHR 123. Molecular Formula: C21H18N2O3. Formula Weight: 346.4. Purity: >98%. Emission: 536 nm. Excitation: 500 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: 0.1 M HCl: 10 mg/ml, DMF: 10 mg/ml, DMSO: 10 mg/ml, Ethanol: 10 mg/ml. lambdamax: 223, 289 nm. SMILES: COC(=O)c1ccccc1C1c2ccc(N)cc2Oc2cc(N)ccc12. InChi Code: InChI=1S/C21H18N2O3/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20/h2-11,20H,22-23H2,1H3. InChi Key: FNEZBBILNYNQGC-UHFFFAOYSA-N.