Digoxigenin maleimide

Digoxigenin (DIG) is a hapten, a small molecule with high antigenicity, which is used in many biological detections similarly to other popular haptens such as 2,4-Dinitrophenol (DNP) and biotin. Typically, digoxigenin is conjugated to a macrobiomolecule (e.g., proteins, nucleic acids, etc) to be detected. Anti-digoxigenin antibodies are labeled with fluorescent dyes or enzymes, directly or secondarily, for visualization and detection. Digoxigenin maleimide readily reacts with thiol group under mild conditions, attaching the digoxigenin moiety to proteins or thiol-substituted oligonucleotides. Digoxigenin-labeled biomolecules are used for biological detections in combination with anti-digoxigenin antibodies of high affinity and specificity.