DDO-5936

DDO-5936 is an inhibitor of the protein-protein interaction between heat shock protein 90 (Hsp90) and cell division cycle 37 (Cdc37). It inhibits the Hsp90-Cdc37 interaction in HCT116 human colorectal cancer cells when used at concentrations of 5, 10, and 25 µM. DDO-5936 is selective for inhibition of the Hsp90-Cdc37 interaction over inhibition of 20 kinases (IC50s = >100 µM for all) and inhibits the proliferation of HCT116 cells (IC50 = 8.99 µM). It also reduces the levels of the Hsp90 kinase client Cdk4 and induces cell cycle arrest at the G0/G1 phase in a Cdc37-dependent manner in HCT116 cells when used at concentrations of 10 and 25 µM. DDO-5936 (50 and 100 mg/kg per day) reduces tumor growth and tumor weight in an HCT116 mouse xenograft model.Formal Name: N-[4-[[2-(1-pyrrolidinyl)-4-pyrimidinyl]amino]phenyl]-N-[(2,4,6-trimethylphenyl)sulfonyl]-glycine. CAS Number: 2355377-13-6. Molecular Formula: C25H29N5O4S. Formula Weight: 495.6. Purity: >98%. Formulation: (Request formulation change), A solid. Solubility: Acetonitrile: Slightly Soluble: 0.1-1 mg/ml, Chloroform: Slightly Soluble: 0.1-1 mg/ml, DMSO: Sparingly Soluble: 1-10 mg/ml. SMILES: O=C(CN(S(=O)(C1=C(C)C=C(C)C=C1C)=O)C2=CC=C(NC3=NC(N4CCCC4)=NC=C3)C=C2)O. InChi Code: InChI=1S/C25H29N5O4S/c1-17-14-18(2)24(19(3)15-17)35(33,34)30(16-23(31)32)21-8-6-20(7-9-21)27-22-10-11-26-25(28-22)29-12-4-5-13-29/h6-11,14-15H,4-5,12-13,16H2,1-3H3,(H,31,32)(H,26,27,28). InChi Key: IIPYRKNROAWEPK-UHFFFAOYSA-N.