DAF-2 diacetate

DAF-2 diacetate is a sensitive fluorescent indicator for the detection and bioimaging of nitric oxide (NO). It is a cell-permeable derivative of DAF-2. Upon entry into the cell, DAF-2 diacetate is transformed into the less cell-permeable DAF-2 by cellular esterases thus preventing loss of signal due to diffusion of the molecule from the cell. In the presence of oxygen, DAF-2 reacts with NO to yield the highly fluorescent triazolofluorescein (DAF-2T). Fluorescence is monitored using excitation and emission wavelengths of 485 and 538 nm, respectively. At neutral pH the detection limit for NO is 2-5 nM. DAF-2 diacetate can be utilized in cells which produce small amounts of NO, such as endothelial cells, as well as in cells which generate large amount of NO, such as macrophages.Formal Name: 2-(3,6-diacetyloxy-4,5-diamino-9H-xanthen-9-yl)-benzoic acid. CAS Number: 205391-02-2. Synonyms: 4,5-Diaminofluorescein diacetate. Molecular Formula: C24H18N2O7. Formula Weight: 446.4. Purity: >95%. Emission: 538 nm. Excitation: 485 nm. Formulation: (Request formulation change), A solution in DMSO. Solubility: DMSO: Soluble. SMILES: NC(C(N)=C1)=CC(C23C4=CC=C(OC(C)=O)C=C4OC5=C2C=CC(OC(C)=O)=C5)=C1C(O3)=O. InChi Code: InChI=1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3. InChi Key: PTSUYDXEEKDBQU-UHFFFAOYSA-N.