Columbianadin

Columbianadin is a coumarin that has been found in A. pubescens and has diverse biological activities. It inhibits depolarization-induced calcium uptake in GH3 rat pituitary cells. Columbianadin inhibits HIV-1 replication in H9 cells (EC50 = 4.6 µM). It inhibits IL-1beta-induced production of IL-6 in A549 cells and reduces inducible nitric oxide synthase (iNOS) levels and LPS-induced NO production in MH-S cells. Columbianadin (20-60 mg/kg) decreases the number of alveolar and interstitial macrophages and alveolar wall thickness in a mouse model of LPS-induced lung inflammation. It also reduces formalin-induced paw licking in mice when administered at a dose of 10 mg/kg.Formal Name: (2Z)-2-methyl-2-butenoic acid, 1-[(8S)-8,9-dihydro-2-oxo-2H-furo[2,3-h]-1-benzopyran-8-yl]-1-methylethyl ester. CAS Number: 5058-13-9. Synonyms: Columbianetin. Molecular Formula: C19H20O5. Formula Weight: 328.4. Purity: >98%. Formulation: (Request formulation change), A solid. Solubility: DMF: 25mg/mL, DMF:PBS (pH 7.2) (1:4): 0.2mg/mL, DMSO: 20mg/mL, Ethanol: 2mg/mL. lambdamax: 325 nm. SMILES: O=C1C=CC2=C(C(C[C@@H](C(OC(/C(C)=C\C)=O)(C)C)O3)=C3C=C2)O1. InChi Code: InChI=1S/C19H20O5/c1-5-11(2)18(21)24-19(3,4)15-10-13-14(22-15)8-6-12-7-9-16(20)23-17(12)13/h5-9,15H,10H2,1-4H3/b11-5-/t15-/m0/s1. InChi Key: JRIBPWOXWIRQOQ-GHAIFCDISA-N. Origin: Plant/Angelicae pubescentis radix.