Citrulline-specific Probe-Rhodamine

Description: Protein arginine deiminases (PADs) catalyze the posttranslational modification of arginine residues on proteins to form citrulline, which plays a large role in regulating gene expression. Abnormally high PAD activity has been observed in a host of human diseases. Citrulline-specific probe-rhodamine is a highly sensitive, rhodamine phenylglyoxal-based fluorophore that specifically detects protein citrullination via a chemoselective reaction between glyoxal and citrulline. This chemical probe (comprised of a single isomer) is capable of reacting with any citrulline-containing protein and can be analyzed with fluorescent imaging (excitation 532 nm, emission 580 nm). When added at 100 µM for 30 minutes at acidic pH, this probe has a reported limit of detection of ~10 ng for citrullinated histone H3 and ~1 ng for autodeiminated PAD4. Smiles: O=CC(=O)C=1C=CC=C(C1)NC(=O)C(N2N=NC(=C2)CNC(=O)C3=CC=C4C(=C3)C(=O)OC54C6=CC=C(C=C6OC7=CC(=CC=C75)N(C)C)N(C)C)C.O