BTC AM

BTC AM is a cell-permeable acetoxy-methyl ester of BTC (Cay-20423). As BTC AM enters cells, it is hydrolyzed by intracellular esterases to produce BTC. BTC is a low affinity calcium indicator (Kd = 7-26 µM) that displays excitation/emission spectra of 401/529 nm, respectively. It exhibits a shift in the excitation maximum from approximately 480 to 401 nm upon calcium binding, enabling determination of ratiometric calcium measurements. BTC is suitable for detecting elevated calcium levels associated with activation of smooth muscle, neurons, and intracellular calcium stores.Formal Name: N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-N-[3-(2-benzothiazolyl)-6-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]-2-oxo-2H-1-benzopyran-7-yl]-glycine(acetyloxy)methyl ester. CAS Number: 176767-94-5. Molecular Formula: C45H45N3O20S. Formula Weight: 979.9. Purity: >90%. Emission: 529 nm. Excitation: 401 nm (Ca2+-bound), 480 nm. Formulation: (Request formulation change), A solid. Solubility: DMSO: soluble. SMILES: O=C(C(C1=NC2=C(C=CC=C2)S1)=C3)OC4=C3C=C(OCCOC5=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=CC(C)=C5)C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C4. InChi Code: InChI=1S/C45H45N3O20S/c1-26-10-11-34(47(18-40(53)64-22-60-27(2)49)19-41(54)65-23-61-28(3)50)37(14-26)58-12-13-59-38-16-31-15-32(44-46-33-8-6-7-9-39(33)69-44)45(57)68-36(31)17-35(38)48(20-42(55)66-24-62-29(4)51)21-43(56)67-25-63-30(5)52/h6-11,14-17H,1. InChi Key: JHWYIECNPLLVMW-UHFFFAOYSA-N.