Boc-LRR-AMC (trifluoroacetate salt)

Boc-LRR-AMC is a fluorogenic substrate for the trypsin-like activity of the 26S proteasome or 20S proteolytic core. Upon enzymatic cleavage by the 26S proteasome or 20S proteolytic core, amino-4-methylcoumarin (AMC) is released and its fluorescence can be used to quantify 26S proteasome or 20S proteolytic core trypsin-like activity. AMC displays excitation/emission maxima of 340-360/440-460 nm, respectively.Formal Name: N-[(1,1-dimethylethoxy)carbonyl]-L-leucyl-L-arginyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-L-argininamide, trifluoroacetate salt. Synonyms: Boc-Leu-Arg-Arg-AMC, Boc-Leu-Arg-Arg-7-amido-4-Methylcoumarin. Molecular Formula: C33H52N10O7 . XCF3COOH. Formula Weight: 700.8. Purity: >90%. Emission: 440-460 nm. Excitation: 340-360 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 14 mg/ml, DMSO: 12 mg/ml, Ethanol: 10 mg/ml, PBS (pH 7.2): 1 mg/ml. lambdamax: 328 nm. SMILES: O=C1C=C(C)C2=C(C=C(NC([C@H](CCCNC(N)=N)NC([C@H](CCCNC(N)=N)NC([C@H](CC(C)C)NC(OC(C)(C)C)=O)=O)=O)=O)C=C2)O1.FC(F)(F)C(O)=O. InChi Code: InChI=1S/C33H52N10O7.C2HF3O2/c1-18(2)15-24(43-32(48)50-33(4,5)6)29(47)42-23(10-8-14-39-31(36)37)28(46)41-22(9-7-13-38-30(34)35)27(45)40-20-11-12-21-19(3)16-26(44)49-25(21)17-20,3-2(4,5)1(6)7/h11-12,16-18,22-24H,7-10,13-15H2,1-6H3,(H,40,45)(H,41,46)(H,42,47)(H,43,48)(H4,34,35,38)(H4,36,37,39),(H,6,7)/t22-,23-,24-,/m0./s1. InChi Key: WBHVYNXRSPOHJK-NYTZCTPBSA-N.