Biocytin

Biocytin is a conjugate of biotin and lysine known formally as ?-N-(d-biotinyl)-L-lysine. The addition of lysine to biotin increases the chain length extending from biotin, allowing the synthesis of medium-chain reagents. It also provides terminal carboxyl and amino groups for derivatization or conjugation to proteins and other molecules. Covalent linking reactions typically involve carbodiimide or NHS-ester crosslinking chemistries. Biocytin is also used as an anterograde, retrograde, or intracellular neuroanatomical tracer that is fixable with aldehyde-based fixatives. For this and other applications, biocytin is detected with avidin or streptavidin probes.Formal Name: N6-[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]-L-lysine. CAS Number: 576-19-2. Synonyms: ?-N-(d-Biotinyl)-L-lysine. Molecular Formula: C16H28N4O4S. Formula Weight: 372.5. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: PBS (pH 7.2): 2 mg/ml. SMILES: O=C1N[C@@]2([H])[C@@](CS[C@H]2CCCCC(NCCCC[C@@H](N)C(O)=O)=O)([H])N1. InChi Code: InChI=1S/C16H28N4O4S/c17-10(15(22)23)5-3-4-8-18-13(21)7-2-1-6-12-14-11(9-25-12)19-16(24)20-14/h10-12,14H,1-9,17H2,(H,18,21)(H,22,23)(H2,19,20,24)/t10-,11+,12+,14+/m1/s1. InChi Key: BAQMYDQNMFBZNA-UHXUPSOCSA-N.