BCECF

BCECF is a fluorescent probe commonly used to measure pH. It is a dual-excitation ratiometric pH indicator with a pKa of ~6.98. Measurements of pH are made by determining the ratio of emission intensity, detected at 535 nm, when excited at 490 nm versus the emission intensity when excited at 440 nm. BCECF is membrane impermeable, whereas the acetoxymethyl ester, BCEBF-AM (Cay-15922) is membrane permeant.Formal Name: 5(or 6)-carboxy-3',6'-dihydroxy-3-oxo-spiro[isobenzofuran-1(3H),9'-[9H]xanthene]-2',7'-dipropanoic acid. CAS Number: 85138-49-4. Synonyms: 2',7'-bis(carboxyethyl)-5(6)-Carboxyfluorescein. Molecular Formula: C27H20O11. Formula Weight: 520.4. Purity: >90%. Emission: 535 nm. Excitation: 440, 490 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 5 mg/ml, DMSO: 15 mg/ml, DMSO:PBS (pH 7.2) (1:2): 0.25 mg/ml, Ethanol: 5 mg/ml. lambdamax: 210, 228, 280, 460, 491 nm. SMILES: OC1=C(CCC(O)=O)C=C(C2(C(C=CC(C(O)=O)=C3)=C3C(O2)=O)C(C=C(CCC(O)=O)C(O)=C4)=C4O5)C5=C1.OC6=C(CCC(O)=O)C=C(C7(C(C=C(C(O)=O)C=C8)=C8C(O7)=O)C(C=C(CCC(O)=O)C(O)=C9)=C9O%10)C%10=C6. InChi Code: InChI=1S/2C27H20O11/c28-19-10-21-17(8-12(19)2-5-23(30)31)27(16-4-1-14(25(34)35)7-15(16)26(36)38-27)18-9-13(3-6-24(32)33)20(29)11-22(18)37-21,28-19-10-21-17(7-12(19)2-5-23(30)31)27(16-9-14(25(34)35)1-4-15(16)26(36)38-27)18-8-13(3-6-24(32)33)20(29)11-2. InChi Key: RZFSVSQVNQXHLI-UHFFFAOYSA-N.