Ara-G

Ara-G is an analog of the nucleoside guanosine and an active metabolite of nelarabine (Cay-20248). Ara-G accumulates in T lymphoblasts and malignant T-lymphoid cells, where it is phosphorylated to produce ara-GTP and incorporated into the DNA. Ara-G inhibits DNA replication by 92% after 30 minutes when used at a concentration of 50 µM in CEM cells, which are used as a model for human T lymphoblasts. It also halts the cell cycle at the sub-G1 phase and induces apoptosis in CEM cells. Syngeneic bone marrow containing 6C3HED tumor cells treated with ara-G (100 mM) ex vivo prior to transplantation increases survival of lethally irradiated mice and induces reconstitution of lymphoid, myeloid, and erythroid cell linages.Formal Name: 2-amino-9-beta-D-arabinofuranosyl-1,9-dihydro-6H-purin-6-one. CAS Number: 38819-10-2. Synonyms: ara-Guanosine, Guanine Arabinoside, NSC 76352. Molecular Formula: C10H13N5O5. Formula Weight: 283.2. Purity: >95%. Formulation: (Request formulation change), A solid. Solubility: DMF: 3 mg/ml, DMSO: 5 mg/ml, Ethanol: Partially soluble, PBS (pH 7.2): 0.3 mg/ml. lambdamax: 253 nm. SMILES: O[C@H]1[C@H](O)[C@H](N2C=NC3=C2NC(N)=NC3=O)O[C@@H]1CO. InChi Code: InChI=1S/C10H13N5O5/c11-10-13-7-4(8(19)14-10)12-2-15(7)9-6(18)5(17)3(1-16)20-9/h2-3,5-6,9,16-18H,1H2,(H3,11,13,14,19)/t3-,5-,6+,9-/m1/s1. InChi Key: NYHBQMYGNKIUIF-FJFJXFQQSA-N.