Aconine

Aconine is an alkaloid originally isolated from Aconitum species and active metabolite of aconitine. It inhibits osteoclast differentiation of RANKL-stimulated RAW 264.7 cells and bone resorption in a pit formation assay in a concentration-dependent manner. Aconine also inhibits RANKL-induced activation of NF-kappaB and NFATc1 in RAW 264.7 cells. In vivo, aconine is toxic to mice when administered intravenously at a dose of 120 mg/kg. It induces flaccid paralysis and toxicity in rats with toxic dose (TD50) and LD50 values of 1.5 and 1.7 µmol per animal, respectively.Formal Name: (1alpha,3alpha,6alpha,14alpha,15alpha,16beta)-20-ethyl-1,6,16-trimethoxy-4-(methoxymethyl)-aconitane-3,8,13,14,15-pentol. CAS Number: 509-20-6. Molecular Formula: C25H41NO9. Formula Weight: 499.6. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 25 mg/ml, DMSO: 20 mg/ml, Ethanol: 25 mg/ml, PBS (pH 7.2): 10 mg/ml. SMILES: CO[C@@H]1C2(C3N4CC)[C@@]([C@@H](OC)C3[C@]([C@@H](O)[C@H](OC)[C@]5(O)[C@@H]6O)(O)[C@]6([H])[C@]2(C5)[H])([H])[C@](COC)(C4)[C@H](O)C1. InChi Code: InChI=1S/C25H41NO9/c1-6-26-9-22(10-32-2)12(27)7-13(33-3)24-11-8-23(30)19(28)14(11)25(31,20(29)21(23)35-5)15(18(24)26)16(34-4)17(22)24/h11-21,27-31H,6-10H2,1-5H3/t11-,12-,13+,14-,15?,16+,17-,18?,19-,20+,21+,22+,23-,24?,25-/m1/s1. InChi Key: SQMGCPHFHQGPIF-FGBFNOOXSA-N. Origin: Plant/Aconitum carmichaelii Debx.