ABTS diammonium salt

ABTS (2,2' -Azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid), diammonium salt) is a very sensitive chromogenic peroxidase substrate (including horseradish peroxidase (HRP)) widely used for ELISA applications. The substrate yields a water-soluble green colored product that can be quantified by colorimetric detection at 405 nm. The reaction may be stopped with 1% sodium dodecyl sulfate (SDS). It has commonly been used to assess antioxidant capacity in the Trolox equivalent antioxidant capacity (TEAC) assay. It has a blue color in the presence of sodium persulfate or metmyoglobin but decolorizes upon incubation with antioxidants, and the antioxidant capacity can be determined spectrophotometrically. ABTS is significantly used by the food industry, to measure the antioxidant capacities within different foods. ABTS is also used to monitor glucose concentrations in different solutions including blood serum and as a safe alternative to DAB for the enhancement of blood fingerprints.