ABD957

ABD957 is an inhibitor of alpha/beta-hydrolase domain-containing protein 17 (ABHD17, IC50 = 0. µM for ABHD17B). It is selective for ABHD17A-C over a panel of 52 serine hydrolases, including the depalmitoylases ABHD10, palmitoyl-protein thioesterase 1 (PPT1), and lysophospholipase 1 (LYPLA1) and LYPLA2, at 10 µM but does inhibit ABHD6, ABHD11, ABHD13, and carboxylesterase 1 (CES1) and CES2 at the same concentration. ABD957 increases the palmitoylation of N-Ras in OCI-AML-3 acute myeloid leukemia (AML) cells expressing GFP-N-RasG12D (EC50 = 29 nM). It also increases the plasma membrane localization of N-Ras in the same cells when used at a concentration of 500 nM. ABD957 inhibits the growth of OCI-AML-3 cells in a concentration-dependent manner. InChI: InChI=1S/C27H36F3N7O5S/c1-18-13-33(10-11-35(18)26(40)36-9-8-24(31-36)43(41,42)32(2)3)14-19-4-5-20(27(28,29)30)12-23(19)37-21-6-7-22(37)16-34(15-21)17-25(38)39/h4-5,8-9,12,18,21-22H,6-7,10-11,13-17H2,1-3H3,(H,38,39)/t18-,21?,22?/m0/s1, InChIKey: ISNXAOFEMNFJCO-XTWGIRIWSA-N, SMILES: C[C@H](N(CC1)C(N2N=C(C=C2)S(N(C)C)(=O)=O)=O)CN1CC3=C(N4C5CCC4CN(CC(O)=O)C5)C=C(C(F)(F)F)C=C3