8-Hydroxypyrene-1,3,6-trisulfonic Acid (sodium salt)

8-Hydroxypyrene-1,3,6-trisulfonic acid (HPTS) is a cell-impermeable fluorescent pH probe. It displays excitation maxima of 403 and 450 nm, the intensities of which decrease and increase, respectively, as pH increases from 5 to 9 and an emission maximum of 510 nm. HPTS has been used in excitation ratio imaging to monitor changes in and measure the pH of endocytosed liposomes, acidic organelles, or the cytoplasm in live cell applications. It is sensitive to fluorescence quenching by viologens, which can be reversed in the presence of glucose or other monosaccharides, and has been used as a glucose and carbohydrate sensor.Formal Name: 8-hydroxy-1,3,6-pyrenetrisulfonic acid, trisodium salt. CAS Number: 6358-69-6. Synonyms: HPTS, Pyranine. Molecular Formula: C16H7O10S3 . 3Na. Formula Weight: 524.4. Purity: >95%. Emission: 510 nm. Excitation: 403 and 450 nm, the intensities of which decrease and increase, respectively, as pH increases from 5 to 9. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 1 mg/ml, DMSO: 15 mg/ml, PBS (pH 7.2): 10 mg/ml. lambdamax: 247, 290, 370, 404 nm. SMILES: [O-]S(C1=CC(S([O-])(=O)=O)=C2C3=C4C(C=C2)=C(S([O-])(=O)=O)C=C(O)C4=CC=C31)(=O)=O.[Na+].[Na+].[Na+]. InChi Code: InChI=1S/C16H10O10S3.3Na/c17-11-5-12(27(18,19)20)8-3-4-10-14(29(24,25)26)6-13(28(21,22)23)9-2-1-7(11)15(8)16(9)10,,,/h1-6,17H,(H,18,19,20)(H,21,22,23)(H,24,25,26),,,/q,3*+1/p-3. InChi Key: KXXXUIKPSVVSAW-UHFFFAOYSA-K.