7-Amino-4-methylcoumarin

7-Amino-4-methylcoumarin (AMC) is a fluorescent probe for peptide labeling commonly used in the study of proteases. Conjugation of AMC via its amino group to a peptide substrate results in quenching of the fluorescent signal. Upon enzymatic cleavage of the peptide by proteases, AMC is released and its fluorescence can be used to quantify enzyme activity. AMC displays excitation/emission maxima of 345/445 nm, respectively.Formal Name: 7-amino-4-methyl-2H-1-benzopyran-2-one. CAS Number: 26093-31-2. Synonyms: AMC, Coumarin 120, 4-methyl-7-aminocoumarin, NSC 45796. Molecular Formula: C10H9NO2. Formula Weight: 175.2. Purity: >98%. Emission: 445 nm. Excitation: 345 nm. Formulation: (Request formulation change), A solid. Solubility: DMF: 3 mg/ml, DMF:PBS (pH 7.2) (1:50): 0.01 mg/ml, DMSO: 3 mg/ml, DMSO:PBS (pH 7.2) (1:40): 0.02 mg/ml. lambdamax: 233, 354 nm. SMILES: O=C(C=C1C)OC2=C1C=CC(N)=C2. InChi Code: InChI=1S/C10H9NO2/c1-6-4-10(12)13-9-5-7(11)2-3-8(6)9/h2-5H,11H2,1H3. InChi Key: GLNDAGDHSLMOKX-UHFFFAOYSA-N.