5-IAF

5-IAF is a fluorescent probe for labeling proteins. It has been used to label proteins through a reaction with sulfhydryl groups. 5-IAF displays excitation/emission maxima of 491/518 nm, respectively, which can shift during protein labeling or changes in hydrophobicity and can be quenched by potassium iodide. 5-IAF has been used to monitor ligand binding and conformational changes of the Na+/K+-ATPase.Formal Name: N-(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl)-2-iodo-acetamide. CAS Number: 63368-54-7. Synonyms: 5-Iodoacetamidofluorescein. Molecular Formula: C22H14INO6. Formula Weight: 515.3. Purity: >90%. Emission: 518 nm. Excitation: 491 nm. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 30 mg/ml, DMSO: 30 mg/ml, DMSO:PBS (pH 7.2) (1:3): 0.25 mg/ml, Ethanol: 500µg/ml. lambdamax: 227, 275, 457 nm. SMILES: OC1=CC=C(C2(C(C=CC(NC(CI)=O)=C3)=C3C(O2)=O)C(C=CC(O)=C4)=C4O5)C5=C1. InChi Code: InChI=1S/C22H14INO6/c23-10-20(27)24-11-1-4-15-14(7-11)21(28)30-22(15)16-5-2-12(25)8-18(16)29-19-9-13(26)3-6-17(19)22/h1-9,25-26H,10H2,(H,24,27). InChi Key: UATCLPJEZJKNHE-UHFFFAOYSA-N.