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4-Thiouracil is a site-specific, photoactivatable probe used to detect RNA structures and nucleic acid-nucleic acid contacts. It absorbs ultraviolet light >300 nm and, in the presence of oxygen, acts as an energy donor to produce singlet oxygen by triplet-triplet energy transfer. The highly reactive oxygen species then reacts readily with 4-thiouracil, leading to the production of uracil and uracil-6-sulfonate, which is fluorescent at a wavelength of ~390 nm. 4-Thiouracil is used as a T. gondii uracil phosphoribosyltransferase substrate to produce 4-thiouridine monophosphate, which can ultimately be incorporated into RNA.Formal Name: 3,4-dihydro-4-thioxo-2(1H)-pyrimidinone. CAS Number: 591-28-6. Synonyms: 2-hydroxy-4-Mercaptopyrimidine, NSC 43288, 4-Thiopyrimidin-2-one, 4-TU. Molecular Formula: C4H4N2OS. Formula Weight: 128.1. Purity: >98%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 10 mg/ml, DMF:PBS (pH 7.2) (1:5): 0.5 mg/ml, DMSO: 10 mg/ml, Ethanol: 1 mg/ml. lambdamax: 246, 326 nm. SMILES: O=C(NC=C1)NC1=S. InChi Code: InChI=1S/C4H4N2OS/c7-4-5-2-1-3(8)6-4/h1-2H,(H2,5,6,7,8). InChi Key: OVONXEQGWXGFJD-UHFFFAOYSA-N.
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the usability of this website, serve for direct advertising or simplify interaction with other websites and social networks, will only be used with your consent.
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