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Product information "4-Methylumbelliferyl-beta-D-Glucuronide (hydrate)"
4-Methylumbelliferyl-beta-D-glucuronide is a fluorogenic substrate of beta-glucuronidase. 4-Methylumbelliferyl-beta-D-glucuronide is cleaved by beta-glucuronidase to release the fluorescent moiety 4-methylumbelliferyl (4-MU). 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nm, increasing as pH decreases. 4-Methylumbelliferyl-beta-D-glucuronide has been used in the beta-glucuronidase reporter system, also known as the GUS reporter system, for fluorescent detection of beta-glucuronidase gene expression in E. coli and transformed plants.Formal Name: 4-methyl-2-oxo-2H-1-benzopyran-7-yl-beta-D-glucopyranosiduronic acid, dihydrate. Synonyms: 4-Methylumbelliferyl-beta-D-Glucopyranosiduronic Acid, MUG. Molecular Formula: C16H16O9 . 2H2O. Formula Weight: 388.3. Purity: >95%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 20 mg/ml, DMSO: 5 mg/ml, Ethanol: 2 mg/ml, PBS (pH 7.2): 2 mg/ml. lambdamax: 319 nm. SMILES: O[C@H]1[C@H](OC2=CC=C(C(C)=CC(O3)=O)C3=C2)O[C@H](C(O)=O)[C@@H](O)[C@@H]1O.O.O. InChi Code: InChI=1S/C16H16O9.2H2O/c1-6-4-10(17)24-9-5-7(2-3-8(6)9)23-16-13(20)11(18)12(19)14(25-16)15(21)22,,/h2-5,11-14,16,18-20H,1H3,(H,21,22),2*1H2/t11-,12-,13+,14-,16+,,/m0../s1. InChi Key: WRVAYRGWFCRSBR-CMNHCFHDSA-N.
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