4-Methylumbelliferyl-alpha-L-Iduronide (free acid)

4-Methylumbelliferyl-alpha-L-iduronide (free acid) is a fluorogenic substrate for alpha-L-iduronidase, an enzyme found in cell lysosomes that is involved in the degradation of glycosaminoglycans such as dermatan sulfate and heparin sulfate. 4-Methylumbelliferyl-alpha-L-iduronide is cleaved by alpha-L-iduronidase to release the fluorescent moiety 4-methylumbelliferyl (4-MU). 4-MU fluorescence is pH-dependent with excitation maxima of 320 and 360 nm at low (1.97-6.72) and high (7.12-10.3) pH, respectively, and an emission maximum ranging from 445 to 455 nM, increasing as pH decreases. This substrate is used in assays that measure the activity of alpha-L-iduronidase, which is commonly deficient in a type of lysosomal storage disease called mucopolysaccharidosis.Formal Name: 4-methyl-2-oxo-2H-1-benzopyran-7-yl alpha-L-idopyranosiduronic acid. CAS Number: 66966-09-4. Synonyms: 4-Methylumbelliferyl-alpha-L-Idopyranosiduronic Acid, 4-MU-alpha-IdoA, MU-alpha-IdoA. Molecular Formula: C16H16O9. Formula Weight: 352.3. Purity: >90%. Formulation: (Request formulation change), A crystalline solid. Solubility: DMF: 20 mg/ml, DMSO: 5 mg/ml, Ethanol: 1 mg/ml, PBS (pH 7.2): 1 mg/ml. lambdamax: 216, 318 nm. SMILES: O=C1C=C(C)C2=C(C=C(O[C@H]3[C@H](O)[C@@H](O)[C@H](O)[C@H](C(O)=O)O3)C=C2)O1. InChi Code: InChI=1S/C16H16O9/c1-6-4-10(17)24-9-5-7(2-3-8(6)9)23-16-13(20)11(18)12(19)14(25-16)15(21)22/h2-5,11-14,16,18-20H,1H3,(H,21,22)/t11-,12-,13+,14+,16+/m0/s1. InChi Key: ARQXEQLMMNGFDU-ZHMBSYLPSA-N.