2,7-Dichlorodihydrofluorescein diacetate

2,7-Dichlorodihydrofluorescein diacetate
Item number Size Datasheet Manual SDS Delivery time Quantity Price
Cay85155-50 50 mg -

6 - 10 business days*

Cay85155-100 100 mg -

6 - 10 business days*

Cay85155-250 250 mg -

6 - 10 business days*

Cay85155-500 500 mg -

6 - 10 business days*

DCFH (commonly known as dichlorofluorescin) is used as an indicator of peroxynitrite formation.... more
Product information "2,7-Dichlorodihydrofluorescein diacetate"
DCFH (commonly known as dichlorofluorescin) is used as an indicator of peroxynitrite formation. DCFH is supplied as the diacetate ester. Following enzymatic or base-catalyzed cleavage of the diacetate groups, it is readily oxidized to the highly fluorescent product dichlorofluorescein (DHF). Peroxynitrite is an efficient mediator of this oxidation and neither NO, superoxide, nor hydrogen peroxide alone appear to oxidize DCFH. Formation of DHF can be monitored by fluorescence spectroscopy using excitation and emission wavelengths of 502 and 523 nm, respectively, or by absorbance spectroscopy at 500 nm (? = 59,500 M-1cm-1). Formal Name: 2-(2,7-dichloro-3,6-diacetyloxy-9H-xanthen-9-yl)-benzoic acid. CAS Number: 4091-99-0. Synonyms: DCFH, DCFH-DA. Molecular Formula: C24H16Cl2O7. Formula Weight: 487.3. Purity: >95%. Emission: 523 nm. Excitation: 502 nm. Formulation: A crystalline solid. Solubility: 0.1 M Na2CO3: 5 mg/ml, DMF: >25 mg/ml, DMSO: >33 mg/ml, Ethanol: >25 mg/ml, PBS pH 7.2: <20 µg/ml. lambdamax: 258 nm. SMILES: CC(=O)Oc1cc2Oc3cc(OC(=O)C)c(Cl)cc3C(c2cc1Cl)c1ccccc1C(=O)O. InChi Code: InChI=1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30). InChi Key: PXEZTIWVRVSYOK-UHFFFAOYSA-N.
Keywords: DCFH, DCFH-DA, 2-(2,7-dichloro-3,6-diacetyloxy-9H-xanthen-9-yl)-benzoic acid
Supplier: Cayman Chemical
Supplier-Nr: 85155


Application: Peroxynitrite indicator
MW: 487.3 D
Formula: C24H16Cl2O7
Purity: >95%
Format: Crystalline Solid

Database Information

CAS : 4091-99-0| Find alternatives

Handling & Safety

Storage: -20°C
Shipping: +20°C (International: -20°C)
Signal Word: Warning
GHS Hazard Pictograms:
H Phrases: H315, H319, H335
P Phrases: P261, P264, P271, P280, P302+352, P304+340, P305+351+338, P312, P321, P332+313, P337+313, P362+364, P403+233, P405, P501
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