Actinomycin D

Suitable for use in nucleic acid inhibition. Actinomycin D inhibits the proliferation of cells in a cell-cycle nonspecific way by forming a stable complex with DNA (via deoxyguanosine residues), thus inhibiting DNA-primed RNA synthesis. It is used in cell culture as a selection agent. Antibiotic. Antitumor compound. Cytotoxic. Apoptosis inducer. RNA synthesis inhibitor. DNA intercalating agent. Mcl-1 expression inhibitor. p53 pathway activator. Source: Streptomyces sp. Synonyms: Streptomyces antibioticus, 2-Amino-(N,N)-1-bis(hexadecahydro-6­,13-diisopropyl-2,5,9-trimethyl-1,4,7,11,14-pentao­xo-1H-pyrrolo[2,1]-[1,4,7,10,13]oxatetraazacyclohexadecin-10-yl)-4,6-dimethyl-3-ox­o-3H-phenoxazine-1,9-dicarboxamide, Dactinomycin, Actinomycin IV, Actinomycin C1, Actactinomycin A IV, Actinomycin 7, Dilactone Actinomycindioic D Acid, Lyovac Cosmegen, Cosmogen, Meractinomycin, CAS No: 50-76-0, Molecular Formula: C62H86N12O16, Molecular Weight: 1255.43, Appearance: Orange to bright red crystaline powder, Identity: Conforms to USP35, A. Ultraviolet absorption, Ratio: A240/A445 between 1.30 to1.50, B. HPLC Conform, Potency: 950~1030ug/mg, Specific Rotation: -293°~-329°, Melting Point: 243~248?, Crystallinity: Conforms, Loss on Drying (2h,105ºC): <5%, Endotoxin: <100EU/mg, RNase: None Detected, DNase: None Detected, Protease: None Detected, Working Concentration: 0.01 to 2ug/ml, Solublility: Freely soluble in alcohol,soluble in water at 10°C, slightly soluble in water at 37°C, very, slightly soluble in ether, Dimethyl sulfoxide (DMSO): 1-50mg/ml, Handling: Light and moisture sensitive. Storage: 4°C